add 优化R脚本
This commit is contained in:
parent
16cd091932
commit
1fce34b1ca
@ -1,27 +1,27 @@
|
|||||||
# Load required packages
|
# 加载所需的包
|
||||||
suppressPackageStartupMessages(library(circlize))
|
suppressPackageStartupMessages(library(circlize))
|
||||||
suppressPackageStartupMessages(library(RColorBrewer))
|
suppressPackageStartupMessages(library(RColorBrewer))
|
||||||
suppressPackageStartupMessages(library(ComplexHeatmap))
|
suppressPackageStartupMessages(library(ComplexHeatmap))
|
||||||
suppressPackageStartupMessages(library(dendextend))
|
suppressPackageStartupMessages(library(dendextend))
|
||||||
|
|
||||||
# Get arguments from the command line
|
# 获取命令行参数
|
||||||
args <- commandArgs(trailingOnly = TRUE)
|
args <- commandArgs(trailingOnly = TRUE)
|
||||||
|
|
||||||
# Check the number of arguments
|
# 检查参数数量
|
||||||
if (length(args) != 2) {
|
if (length(args) != 2) {
|
||||||
stop("Please provide two arguments: the input CSV file path and the output PDF file path.", call. = FALSE)
|
stop("请提供两个参数:输入CSV文件路径和输出PDF文件路径。", call. = FALSE)
|
||||||
}
|
}
|
||||||
|
|
||||||
# Get the input CSV file path and the output PDF file path
|
# 获取输入CSV文件路径和输出PDF文件路径
|
||||||
input_csv <- args[1]
|
input_csv <- args[1]
|
||||||
output_pdf <- args[2]
|
output_pdf <- args[2]
|
||||||
|
|
||||||
# Check if the input file exists
|
# 检查输入文件是否存在
|
||||||
if (!file.exists(input_csv)) {
|
if (!file.exists(input_csv)) {
|
||||||
stop("The input file does not exist. Please check the file path.", call. = FALSE)
|
stop("输入文件不存在。请检查文件路径。", call. = FALSE)
|
||||||
}
|
}
|
||||||
|
|
||||||
# Function to load and preprocess data
|
# 函数加载和预处理数据
|
||||||
load_and_preprocess_data <- function(file_path) {
|
load_and_preprocess_data <- function(file_path) {
|
||||||
data <- read.table(file = file_path, header = TRUE, row.names = 1, sep = ',')
|
data <- read.table(file = file_path, header = TRUE, row.names = 1, sep = ',')
|
||||||
data_matrix <- as.matrix(data)
|
data_matrix <- as.matrix(data)
|
||||||
@ -30,31 +30,37 @@ load_and_preprocess_data <- function(file_path) {
|
|||||||
return(normalized_data)
|
return(normalized_data)
|
||||||
}
|
}
|
||||||
|
|
||||||
# Load and preprocess data
|
# 加载和预处理数据
|
||||||
cir1 <- load_and_preprocess_data(input_csv)
|
cir1 <- load_and_preprocess_data(input_csv)
|
||||||
|
|
||||||
# Define the color gradient for the heatmap
|
# 打印数据维度和头部
|
||||||
|
print(dim(cir1))
|
||||||
|
print(head(cir1))
|
||||||
|
|
||||||
|
# 定义颜色梯度
|
||||||
mycol <- colorRamp2(c(-2.5, 0.3, 3.1), c("blue", "white", "red"))
|
mycol <- colorRamp2(c(-2.5, 0.3, 3.1), c("blue", "white", "red"))
|
||||||
|
|
||||||
# Open the PDF device
|
# 打开PDF设备,设置宽度和高度相等
|
||||||
pdf(output_pdf, width = 10, height = 8)
|
pdf(output_pdf, width = 8, height = 8)
|
||||||
|
|
||||||
# Draw the adjusted and beautified circular heatmap
|
# 设置绘图参数
|
||||||
circos.par(gap.after = c(30))
|
circos.par(gap.after = c(30))
|
||||||
|
|
||||||
|
# 绘制调整后的圆形热图
|
||||||
circos.heatmap(cir1,
|
circos.heatmap(cir1,
|
||||||
col = mycol,
|
col = mycol,
|
||||||
dend.side = "inside",
|
dend.side = "inside",
|
||||||
rownames.side = "outside",
|
rownames.side = "outside",
|
||||||
rownames.col = "black",
|
rownames.col = "black",
|
||||||
rownames.cex = 1.3,
|
rownames.cex = 1.3,
|
||||||
track.height = 0.35,
|
track.height = 0.35, # 增加轨道高度
|
||||||
cluster = TRUE,
|
cluster = TRUE,
|
||||||
dend.track.height = 0.18,
|
dend.track.height = 0.18,
|
||||||
dend.callback = function(dend, m, si) {
|
dend.callback = function(dend, m, si) {
|
||||||
color_branches(dend, k = 15, col = 1:15)
|
color_branches(dend, k = 15, col = 1:15)
|
||||||
})
|
})
|
||||||
|
|
||||||
# Add column names
|
# 添加列名
|
||||||
circos.track(track.index = get.current.track.index(), panel.fun = function(x, y) {
|
circos.track(track.index = get.current.track.index(), panel.fun = function(x, y) {
|
||||||
if (CELL_META$sector.numeric.index == 1) {
|
if (CELL_META$sector.numeric.index == 1) {
|
||||||
cn <- colnames(cir1)
|
cn <- colnames(cir1)
|
||||||
@ -65,14 +71,14 @@ circos.track(track.index = get.current.track.index(), panel.fun = function(x, y)
|
|||||||
}
|
}
|
||||||
}, bg.border = NA)
|
}, bg.border = NA)
|
||||||
|
|
||||||
# Add legend
|
# 添加图例
|
||||||
lg <- Legend(title = "Exp", col_fun = mycol, direction = "vertical")
|
lg <- Legend(title = "Exp", col_fun = mycol, direction = "vertical")
|
||||||
grid.draw(lg)
|
grid.draw(lg)
|
||||||
|
|
||||||
# Clear the circular plot
|
# 清除圆形图
|
||||||
circos.clear()
|
circos.clear()
|
||||||
|
|
||||||
# Close the PDF device
|
# 关闭PDF设备
|
||||||
dev.off()
|
dev.off()
|
||||||
|
|
||||||
message("The heatmap has been successfully saved to: ", output_pdf)
|
message("热图已成功保存至: ", output_pdf)
|
Loading…
x
Reference in New Issue
Block a user